Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Infectious diseases of animals represent a significant economic burden to economies world-wide and may raise relevant Public Health concerns.

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies

Veterinary Infection Biology Molecular Diagnostics And High Throughput Strategies

Molecular diagnostics has emerged as the fastest growing and trending segment of the in vitro diagnostics industry and seems also promising in the veterinary sector, providing novel and powerful tools that enable the fast and accurate diagnosis of animal diseases.

In this book, we provide an overview of molecular biology tools for pathogen detection and characterization, along with potential applications to disclose pathogen biology and interaction with the host. In Part I, bio banking, bio safety and good laboratory practices, biological specimen collection and processing, quality assurance and control, and validation of molecular diagnostic assays for veterinary use are concisely introduced as a basis for the molecular strategies enclosed in protocol sections.

The significance and integration of molecular biology methods in the framework of veterinary practice are discussed, along with a SWOT analysis presenting our view on the advantages, limitations, opportunities and weaknesses, or challenges, of these approaches. The rationale is on the techniques and applications rather than the pathogen or disease models. Part II is dedicated to the molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches.

A brief overview of the advances on nano science and microfluidics is provided, including a discussion on how the recent developments in these research fields may be translated into field deployable biosensors. Part III addresses geno typing tools for assessing the genetic landscape and epidemiology of pathogens. Part IV introduces integrative omics and high-throughput technologies as powerful research tools, yielding massive amounts of information that may ultimately improve the control and management of infectious diseases. We strived to span a broad range of molecular approaches, from simple and affordable to emerging and sophisticated, which we anticipate will be useful for veterinary doctors and laboratory technicians, researchers, and students of veterinary medicine and science.

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